Calcium Regulating Hormones Vitamin D Metabolites and Cyclic AMP Assays and Their Clinical Application

Description

Calcium metabolism is regulated by three specific hormones: parathor mone calciferol metabolites and calcitonin. Cyclic AMP also plays an important part in calcium regulation and its concentration in urine can be taken as a measure of parathyroid function. Methods for quantitative measurement of e.g. calcium regulating hormones and vitamin D metabolites as well as of cAMP are absolutely necessary for safe differential diagnosis of diseases of the parathyroid like hypo and hyperparathyroidism. In this monograph all presently available methods are summarized as to whether they can already be obtained as test kits or are still being tested. Radioimmunoassays for the various peptide fragments such as 53 84 44 68 28 48 and 1 34 are described as are immunochemiluminescence methods and adenylate cyclase bioassays. The same is true for the vitamin D metabolites where the various assays for calcidiol and calcitriol such as HPLC protein binding assay with second antibody separation and double antibody RIA are deA scribed. Finally determination of calcitonin and cAMP is discussed in detail. This is a practically and clinically oriented monograph for working in the fields of internal medicine endocrinology and laboratory medicine.

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